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Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
Nandhinee Radha Shanmugam1, Anjan Panneer Selvam1, Thomas W Barrett2
1Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA; Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA.
Aim:
To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood.
Materials & Methods:
The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy.
Results:
The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml.
Conclusion:
This novel technology has promising preliminary results for rapid and sensitive detection of cTnT.
