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Updated: Mar 9, 2026
Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
MS for investigation of time-dependent protein adsorption on surfaces in complex biological samples
Torgny Undin1, Sara Bergström Lind1, Andreas P Dahlin2
1Department of Chemistry-BMC, Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden; Department of Chemistry-BMC, Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden.
Aim:
This study aims at developing a nondestructive way for investigating protein adsorption on surfaces such as biomaterials using mass spectrometry.
Methods:
Ventricular cerebrospinal fluid in contact with poly carbonate membranes were used as adsorption templates and on-surface enzymatic digestion was applied to desorb proteins and cleave them into peptides. Mass spectrometric analysis provided both protein identification and determination of protein specific adsorption behavior.
Results:
In general, the adsorption increased with incubation time but also protein-specific time-resolved adsorption patterns from the complex protein solution were discovered.
Conclusion:
The method developed is a promising tool for the characterization of biofouling, which sometimes causes rejection and encapsulation of implants and can be used as complement to other surface analytical techniques.
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