A dynamic career in MS: applications to biomedical research
1Penn SRP Center & Center for Excellence in Environmental Toxicology, Department of Systems Pharmacology & Translational Therapeutics, 854 BRB II/III, 421 Curie Blvd, University of Pennsylvania, PA 19104, USA.
Future Science OA
|December 30, 2016
Summary
Ian A. Blair
Area of Science:
- Pharmacology and Environmental Health
- Mass Spectrometry
- Biomarker Development
Background:
- Professor Ian A. Blair has extensive expertise in organic chemistry and pharmacology.
- His career includes significant roles at the University of Pennsylvania, focusing on cancer pharmacology and translational therapeutics.
- He directs a Superfund Research and Training Program Center funded by the National Institute of Environmental Health Sciences (NIEHS).
Purpose of the Study:
- To develop biomarkers for establishing genetic/phenotype correlations.
- To assess the interaction between genes and environmental chemical exposure.
- To investigate the role of cellular oxidative stress in carcinogenesis, cardiovascular disease, and neurodegeneration.
Main Methods:
- Utilizing mass spectrometric methods for structural elucidation and quantification of endogenous biomolecules.
- Developing and applying novel techniques for high-sensitivity quantitative analyses.
- Focusing on electron capture atmospheric pressure chemical ionization for chiral biomolecule analysis.
Main Results:
- Discovery of electron capture atmospheric pressure chemical ionization technique.
- Established expertise in high-sensitivity quantitative analysis of chiral biomolecules.
- Significant contributions to understanding oxidative stress mechanisms in disease.
Conclusions:
- Blair's research advances the understanding of environmental chemical interactions with genetics.
- His work on oxidative stress provides insights into major diseases.
- The developed mass spectrometry techniques enable precise biomolecular quantification.
Related Concept Videos
01:21Tandem Mass Spectrometry
2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
01:19MALDI-TOF Mass Spectrometry
7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K
01:21Mass Spectrometry: Complex Analysis
2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
01:16Mass Spectrometers
9.7K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
9.7K
01:19Mass Spectrometry: Overview
9.6K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.6K
01:33Peptide Identification Using Tandem Mass Spectrometry
8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K