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A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method
Carla Divieto1, Maria Paola Sassi1
1INRIM - Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce, 91 - 10135, Torino, Italy.
Background:
In cell-based therapies, in vitro studies on biomimetic cell-scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell number. Our objective was to develop a nondestructive method for human mesenchymal stem cells dose evaluation in a highly porous scaffold for bone regeneration.
Materials & Measurement Method:
Proliferation trend of human mesenchymal stem cells on Biocoral® scaffolds was measured by a resazurin-based assay here optimized for 3D cultures. The method allows to noninvasively follow the cell proliferation on biocorals over 3 weeks with very high reproducibility.
Conclusion:
This reliable method could be a powerful tool in cell-based therapies for cell dose determination.
