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Tooth tissue engineering: tooth decellularization for natural scaffold.

Luciana Aparecida de Sousa Iwamoto1, Monica Talarico Duailibi2, Gerson Yoshinobu Iwamoto3

  • 1CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil.

Future Science OA
|December 30, 2016
PubMed
Summary

The best method for decellularizing teeth to create natural scaffolds involved using phosphate buffer saline (PBS), ethylenediaminetetraacetic (EDTA), hydrogen peroxide (HP), and enzymatic detergent (ED). This tissue engineering approach preserves natural structure.

Keywords:
decellularizationdemineralizationtissue engineeringtissue scaffold

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Area of Science:

  • Tissue engineering
  • Biomaterials science
  • Regenerative medicine

Background:

  • Tissue engineering seeks to create biological substitutes and replacement organs.
  • Decellularization is a key technique, aiming to remove cellular material while preserving the extracellular matrix structure.
  • Evaluating decellularization methods is crucial for successful scaffold development.

Purpose of the Study:

  • To compare five different decellularization methods for premolar teeth.
  • To identify the most effective method for generating natural scaffolds for tissue engineering applications.

Main Methods:

  • Fifty premolar teeth were divided into five groups with varying decellularization agents: formaldehyde, PBS/EDTA/SH, PBS/EDTA/HP, PBS/EDTA/SH/enzymatic detergent, and PBS/EDTA/HP/enzymatic detergent.
  • Techniques included scanning electron microscopy (SEM), X-ray analysis, weight measurement, and color assessment.
  • Statistical analysis was performed on the collected data.

Main Results:

  • Group 5, utilizing PBS, EDTA, HP, and ED, demonstrated superior results in scaffold preparation.
  • SEM, X-ray, weight, and color analyses indicated the effectiveness of the G5 method.
  • Statistical analysis supported the superiority of the G5 protocol.

Conclusions:

  • The combination of phosphate buffer saline (PBS), ethylenediaminetetraacetic (EDTA), hydrogen peroxide (HP), and enzymatic detergent (ED) proved to be the most effective decellularization strategy.
  • This method is recommended for obtaining natural scaffolds in tissue engineering.
  • The study provides a benchmark for evaluating decellularization protocols.