Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable and rapid hydrazine monitoring by a large Stokes shift probe with smartphone-assisted quantification: Its real-sample applications.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Monitoring alkaline phosphatase activity using blue- and red-emissive biocompatible fluorescent probes in human serum and living cells.

Talanta·2026
Same author

A dual-channel smart fluorescent probe for rapid on-site detection of thiophenol and hydrazine in biological, environmental, and food samples.

Food chemistry·2026
Same author

Design, synthesis, anticancer activity, bioimaging, and molecular docking of novel fluorescent isatin derivatives.

Investigational new drugs·2026
Same author

Electrocardiographic assessment of ventricular repolarization variables in patients with wild-type transthyretin amyloid cardiomyopathy.

Journal of electrocardiology·2026
Same author

Smartphone-integrated NIR fluorescent probe for rapid and sensitive alkaline phosphatase detection in serum and living cells.

Talanta·2026

Related Experiment Video

Updated: Mar 9, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

10.1K

A New Piperidine Derivatized-Schiff Base Based "Turn-on" Cu2+Chemo-Sensor.

Ibrahim Uyanik1, Mehmet Oguz2, Asif Ali Bhatti2,3

  • 1Faculty of Technology, Department of Metallurgical and Materials Engineering, Selcuk University, 42075, Konya, Turkey.

Journal of Fluorescence
|December 30, 2016
PubMed
Summary

A novel Schiff base ligand (L) was synthesized and demonstrated selective fluorescence enhancement for copper(II) ions (Cu2+). This fluorescent probe shows high sensitivity and a 1:1 binding ratio with Cu2+.

Keywords:
Binding constantCopperFluorescencePiperidineSchiff base

More Related Videos

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

3.0K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.8K

Related Experiment Videos

Last Updated: Mar 9, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

10.1K
Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

3.0K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.8K

Area of Science:

  • Coordination Chemistry
  • Fluorescence Spectroscopy
  • Chemical Sensing

Background:

  • Schiff bases are versatile ligands with applications in coordination chemistry and sensing.
  • Developing selective fluorescent probes for metal ions is crucial for environmental and biological monitoring.

Purpose of the Study:

  • To synthesize a new Schiff base ligand, E-1-(((1-benzylpiperidin-4-yl)imino)methyl)naphthalenee-2-ol (L).
  • To evaluate the fluorescence response of the synthesized Schiff base toward copper(II) ions (Cu2+).
  • To investigate the selectivity and binding characteristics of the Schiff base probe for Cu2+.

Main Methods:

  • Synthesis of the Schiff base ligand (L).
  • UV-Visible spectroscopy to analyze solvent effects and metal selectivity.
  • Fluorescence spectroscopy to assess the probe's response to Cu2+.
  • Job's plot and Benesi-Hildebrand analysis to determine binding stoichiometry and constants.

Main Results:

  • The synthesized Schiff base (L) exhibited a significant fluorescence enhancement in the presence of Cu2+.
  • The probe demonstrated high selectivity for Cu2+ over other tested metal ions (Ni2+, Zn2+, Hg2+, Co2+, Cd2+, Al3+, Fe2+, and Pb2+).
  • The binding stoichiometry was determined to be 1:1 (L:Cu2+) with a binding constant (Ka) of 4 × 104 M-1.

Conclusions:

  • The novel Schiff base E-1-(((1-benzylpiperidin-4-yl)imino)methyl)naphthalenee-2-ol (L) is an effective fluorescent probe for Cu2+ detection.
  • The probe's high selectivity and sensitivity make it suitable for potential applications in Cu2+ ion sensing.
  • The detailed investigation provides insights into the coordination chemistry and sensing mechanism of the Schiff base ligand with Cu2+.