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Electrophoretic analysis of proteins from Phycomyces mutants with abnormal tropisms
1Department of Physics, Syracuse University, New York 13244-1130.
Biochemical Genetics
|June 1, 1989
Summary
Mutants of Phycomyces blakesleeanus with defective phototropism (bending responses) show altered protein patterns in microsomal fractions. The madE and madF genes are implicated in regulating these specific protein changes.
Area of Science:
- Mycology
- Molecular Biology
- Genetics
Background:
- Certain phototropism mutants of Phycomyces blakesleeanus exhibit impaired responses to non-light stimuli like gravity and wind.
- These 'stiff' mutants are genetically linked to four specific genes: madD, madE, madF, and madG.
Purpose of the Study:
- To investigate the molecular basis of 'stiff' mutations in Phycomyces blakesleeanus.
- To identify specific protein alterations in microsomal and soluble fractions of wild-type and mutant strains.
Main Methods:
- Utilized two-dimensional gel electrophoresis to analyze protein profiles.
- Examined microsomal and soluble fractions from wild-type, single mutants (madD-madG), and double mutants.
Main Results:
- Consistent protein spot pattern differences were observed in microsomal fractions of madE and madF mutants compared to wild-type.
- In madE mutants, two protein spots (E1 and E2) showed altered abundance, suggesting post-translational regulation by madE.
- A specific protein spot (F1) was present in wild-type but absent in madF mutants, indicating F1 is likely the madF gene product.
Conclusions:
- The madE and madF genes play crucial roles in regulating specific protein expression or modification in Phycomyces blakesleeanus.
- These findings contribute to understanding the molecular mechanisms underlying tropism responses in fungi.
