Related Experiment Video

Updated: Mar 9, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.7K

Expression of MMPs is dependent on the activity of mitogen-activated protein kinase in chondrosarcoma

Min Yao1, Xiaomei Wang1, Yufeng Zhao1

  • 1Department of Pathology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China.

Molecular Medicine Reports
|December 31, 2016
PubMed

Insights

Matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) are elevated in chondrosarcoma, linked to mitogen-activated protein kinase (MAPK) activity. Inhibiting MAPK reduces MMPs, suggesting a therapeutic target for chondrosarcoma progression.

Area of Science:

  • Oncology
  • Biochemistry
  • Molecular Biology

Background:

  • Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) play crucial roles in cartilage homeostasis and disease.
  • Chondrosarcoma, a malignant cartilage tumor, exhibits altered extracellular matrix remodeling.
  • The involvement of mitogen-activated protein kinase (MAPK) signaling in chondrosarcoma progression requires further elucidation.

Purpose of the Study:

  • To investigate the relationship between mitogen-activated protein kinase (MAPK) activity and the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in chondrosarcoma.
  • To determine the role of MMP-1 and MMP-13 in type II collagen degradation and cartilage matrix decomposition in chondrosarcoma.
  • To assess the potential of MAPK inhibitors in modulating MMP expression and chondrosarcoma cell invasion.

Main Methods:

  • Analysis of MMP-1, MMP-13, TIMP-1, type II collagen, and phosphorylated MAPK levels in surgical specimens of normal cartilage, enchondroma, and chondrosarcoma.
  • In vitro experiments using chondrosarcoma cells treated with MAPK inhibitors to assess the impact on MMP and TIMP expression.
  • Quantitative assessment of MMP-1 and MMP-13 expression and their correlation with chondrosarcoma invasion.

Main Results:

  • Expression levels of MMP-1, MMP-13, and TIMP-1 were significantly increased in chondrosarcoma tissues, correlating with elevated MAPK activity.
  • Pretreatment of chondrosarcoma cells with MAPK inhibitors led to a significant reduction in MMP-1, MMP-13, and TIMP-1 levels.
  • MMP-1 and MMP-13 were identified as key enzymes responsible for type II collagen degradation and cartilage matrix decomposition, facilitating chondrosarcoma cell invasion.

Conclusions:

  • MAPK signaling pathway activation is associated with increased expression of MMP-1, MMP-13, and TIMP-1 in chondrosarcoma.
  • MAPK inhibitors effectively suppress the expression of MMPs and TIMPs, offering a potential therapeutic strategy.
  • Targeting the MAPK pathway could be a promising approach to inhibit chondrosarcoma progression by reducing matrix degradation and invasion.

More Related Videos

The In ovo CAM-assay as a Xenograft Model for Sarcoma12:44

The In ovo CAM-assay as a Xenograft Model for Sarcoma

Published on: July 17, 2013

26.4K
Author Spotlight: Genetically Engineered Mouse Models and Pathological Characterization of Neurofibromatosis Type 1 Associated Tumors08:57

Author Spotlight: Genetically Engineered Mouse Models and Pathological Characterization of Neurofibromatosis Type 1 Associated Tumors

Published on: May 17, 2024

2.7K

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.6K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.1K