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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Molecularly Imprinted Membrane Electrospray Ionization for Direct Sample Analyses.

Tianyi Li1,2, Liusheng Fan3, Yingfeng Wang4

  • 1State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China , 102206.

Analytical Chemistry
|December 31, 2016
PubMed
Summary
This summary is machine-generated.

Molecular imprinting integrated with membrane electrospray ionization (MESI) enhances detection sensitivity for complex samples. This novel method improves quantitation limits by 10- to 50-fold for various applications.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Materials Science

Background:

  • Ambient ionization mass spectrometry faces challenges with low detection sensitivity in complex matrices.
  • Direct sample analysis often requires sensitive and selective detection methods.

Purpose of the Study:

  • To integrate molecular imprinting technology with membrane electrospray ionization (MESI) for improved direct sample analysis.
  • To enhance the limit of quantitation for targeted analytes in complex samples.

Main Methods:

  • Preparation of molecularly imprinted membranes (MIMs) by cross-linking a molecularly imprinted polymer onto a polyvinylidene difluoride (PVDF) membrane.
  • Utilizing MIMs for target analyte enrichment prior to mass spectrometry analysis.
  • Validation of the quantitative performance of the MIM-MESI MS methodology.

Main Results:

  • Achieved a 10- to 50-fold improvement in the limit of quantitation compared to conventional methods.
  • Demonstrated satisfactory performance for direct quantification of targeted analytes in complex samples.
  • Verified the recognition characteristics of MIMs for target analytes.

Conclusions:

  • MIM-MESI MS offers a sensitive and effective approach for direct quantification in complex matrices.
  • The target enrichment capability of MIM-MESI MS is applicable to diverse fields like food safety and clinical analysis.
  • This methodology provides a validated tool for sensitive mass spectrometry-based analysis.