Anti-Neuroinflammatory ent-Kaurane Diterpenoids from Pteris multifida Roots
Jung Wha Kim1, Ji Yeon Seo2, Won Keun Oh3
1College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea. jkim11@snu.ac.kr.
Molecules (Basel, Switzerland)
|December 31, 2016
Summary
Researchers identified new compounds from Pteris multifida roots that reduce neuroinflammation. These ent-kaurane diterpenes show potential as anti-neuroinflammatory agents by inhibiting nitric oxide (NO) production in microglia cells.
Area of Science:
- Natural Product Chemistry
- Neuroscience
- Pharmacology
Background:
- Activated microglia are a key source of neuroinflammation, contributing to neurodegenerative diseases like Alzheimer's.
- Phytochemicals are being investigated for their potential to combat neuroinflammation.
Purpose of the Study:
- To isolate and identify bioactive compounds from Pteris multifida roots with anti-neuroinflammatory properties.
- To evaluate the inhibitory effects of these compounds on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated microglia.
Main Methods:
- Extraction of Pteris multifida roots using 80% methanol.
- Isolation and structural elucidation of compounds using NMR, HR-ESI-MS, and CD spectroscopy.
- Assessment of NO inhibitory activity in LPS-activated BV-2 microglia cells.
Main Results:
- Three new ent-kaurane diterpenoids and nine known compounds were isolated.
- Compounds 1 and 7 significantly inhibited NO production in LPS-stimulated BV-2 cells.
- These active compounds reduced the expression of cyclooxygenase-2 (COX-2) and pro-inflammatory mediators (PGE₂, TNF-α, IL-1β, IL-6).
Conclusions:
- Ent-kaurane diterpenes from Pteris multifida roots possess significant anti-neuroinflammatory activity.
- These compounds may serve as potential lead compounds for developing novel therapeutic agents against neuroinflammatory diseases.
