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Related Experiment Videos

High-voltage electron diffraction from bacteriorhodopsin (purple membrane) is measurably dynamical.

R M Glaeser1, T A Ceska

  • 1Biophysics Department, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.

Acta Crystallographica. Section A, Foundations of Crystallography
|September 1, 1989
PubMed
Summary

Dynamical diffraction in bacteriorhodopsin crystals causes observable Friedel differences at lower electron voltages. This finding is crucial for electron crystallography of biological macromolecules.

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Area of Science:

  • Crystallography
  • Biophysics
  • Materials Science

Background:

  • Electron crystallography is vital for determining the structure of biological macromolecules.
  • The kinematic approximation is commonly used, assuming minimal dynamical diffraction effects.
  • Understanding the limits of this approximation is essential for accurate structural determination.

Purpose of the Study:

  • To investigate the impact of electron voltage on electron diffraction patterns of bacteriorhodopsin.
  • To determine the extent of dynamical diffraction in thin crystalline biological samples.
  • To assess the validity of the kinematic approximation in electron crystallography.

Main Methods:

  • Recording electron diffraction patterns of bacteriorhodopsin crystals at 20 kV and 120 kV.

Related Experiment Videos

  • Analyzing Friedel mate intensity differences to detect deviations from kinematic theory.
  • Evaluating potential sources of observed differences, including complex scattering factors and inelastic scattering.

    • Main Results:

    • Significant Friedel differences were observed at 20 kV, diminishing at 120 kV.
    • Dynamical diffraction within the single molecular layer was identified as the cause of these differences.
    • Friedel differences were found to be independent of diffraction resolution.

    Conclusions:

    • Dynamical diffraction effects are significant in biological samples at lower electron voltages.
    • The kinematic approximation may not be universally applicable, especially at lower voltages or for thicker specimens.
    • Exploiting dynamical effects at lower voltages could offer new methods for phasing in electron crystallography.