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Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts.

A Teti, M Grano, S L Teitelbaum

    Bollettino Della Societa Italiana Di Biologia Sperimentale
    |August 1, 1989
    PubMed
    Summary
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    Changes in intracellular pH affect calcium levels in osteoclasts. Acidification decreases calcium, while alkalinization increases it, suggesting pH acts as an intracellular signal modulating osteoclast activity.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Physiology

    Background:

    • Osteoclasts are crucial for bone remodeling.
    • Cytosolic calcium (Ca2+) plays a vital role in regulating cellular functions, including osteoclast activity.
    • The precise mechanisms by which intracellular pH influences Ca2+ levels in osteoclasts are not fully understood.

    Purpose of the Study:

    • To investigate the impact of intracellular pH alterations on cytosolic Ca2+ concentrations in isolated osteoclasts.
    • To elucidate the relationship between pH changes and osteoclast function.

    Main Methods:

    • Single isolated osteoclasts were used.
    • Intracellular alkalinization was induced using a bicarbonate-containing buffer.
    • Intracellular acidification was achieved using a sodium-butyrate-containing buffer.

    Related Experiment Videos

  • Changes in cytosolic Ca2+ were monitored.
  • The effect of VO4 (a Ca2+-ATPase inhibitor) on Ca2+ levels during acidification was assessed.

    • Main Results:

    • Alkalinization led to an increase in cytosolic Ca2+.
    • Acidification resulted in a rapid and sustained decrease in cytosolic Ca2+.
    • The decrease in cytosolic Ca2+ during acidification was partially inhibited by VO4, suggesting Ca2+-ATPase involvement.

    Conclusions:

    • Intracellular pH changes significantly modulate cytosolic Ca2+ levels in osteoclasts.
    • These pH-induced Ca2+ modifications likely act as intracellular signals influencing osteoclast activity.
    • The findings provide insights into the regulation of bone remodeling at the cellular level.