Related Experiment Videos
[Simultaneous analysis of lymphocyte markers using triple color markers]
Bollettino Della Societa Italiana Di Biologia Sperimentale
|August 1, 1989
Summary
This study introduces Streptavidin Duochrome for simultaneous three-color flow cytometry analysis. This new reagent enables quick and easy visualization and quantification of antigen distribution on multiple cell populations.
Area of Science:
- Immunology
- Cell Biology
Context:
- Flow cytometry is a crucial technique for analyzing cell populations.
- Simultaneous multi-color analysis allows for deeper insights into cellular heterogeneity.
Purpose:
- To introduce and evaluate the utility of Streptavidin Duochrome for three-color flow cytometry.
- To assess the importance of this novel analysis for studying lymphocytic population heterogeneity.
Summary:
- Streptavidin Duochrome, a phycoerythrin-Texas red fluorochrome complex, enables simultaneous three-color analysis using FITC, PE, and biotin conjugates with an argon laser.
- Paint-a-gate software facilitates visualization and quantification of single, double, and triple-labeled cells, up to seven combinations.
- Evaluated combinations include CD3/CD4/CD8 for T lymphocytes, CD3/CD19/CD16 for T, B, NK cells, CD4/Leu8/Leu18 for helper subsets, and CD3/CD8/Leu7.
Impact:
- Provides a rapid and easy method for visualizing and quantifying antigen distribution on diverse cell populations.
- Enhances the study of cellular heterogeneity in lymphocytic populations through advanced multi-color analysis.
- Facilitates detailed immunological research by enabling complex cell subset identification.