Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.5K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.6K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlative ultrastructural mapping of Lewy pathology reveals regional diversity in Parkinson's and dementia with Lewy bodies.

Acta neuropathologica·2026
Same author

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules.

Nature communications·2026
Same author

Ultrastructural diversity and subcellular organization of nigral Lewy pathology in Parkinson's disease.

Nature communications·2026
Same author

Structural basis for a filamentous morpheein model of human cystathionine beta-synthase.

Nature communications·2026
Same author

CryoWriter: a robotic solution for improved Cryo-EM grid preparation.

Nature communications·2026
Same author

Temperature-dependent ligand relocation reveals plasticity of TRPM4 inhibition.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Mar 9, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

5.0K

Robust image alignment for cryogenic transmission electron microscopy.

Robert A McLeod1, Julia Kowal1, Philippe Ringler1

  • 1Center for Cellular Imaging and NanoAnalytics, Biozentrum, Universität Basel, Basel, Switzerland.

Journal of Structural Biology
|January 1, 2017
PubMed
Summary
This summary is machine-generated.

Zorro software offers robust drift correction for cryo-electron microscopy image analysis. It improves structure resolution by accurately registering frames in dose-fractionated movies, even with low contrast.

Keywords:
Cross correlationCryogenic transmission electron microscopyDirect detection deviceDose fractionationImage alignmentImage registration

More Related Videos

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

6.1K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

1.2K

Related Experiment Videos

Last Updated: Mar 9, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

5.0K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

6.1K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

1.2K

Area of Science:

  • Structural Biology
  • Microscopy Techniques
  • Computational Biology

Background:

  • Cryo-electron microscopy (cryo-EM) resolution has advanced with direct electron detectors.
  • Dose fractionation in cryo-EM captures movies to correct specimen drift.
  • Existing image registration methods struggle with high noise and low contrast in cryo-EM data.

Purpose of the Study:

  • To present Zorro, a software package for robust drift correction in cryo-EM.
  • To enable reliable frame registration in dose-fractionated movies.
  • To enhance structure resolution in cryo-EM by minimizing drift artifacts.

Main Methods:

  • Developed Zorro software utilizing intensity-normalized cross-correlation.
  • Implemented a logistic noise model for optimal weighting and filtering of cross-correlations.
  • Employed multi-reference (MR) cross-correlation for image registration.
  • Introduced methods for evaluating performance using even/odd frame stacks and Fourier ring correlation.
  • Demonstrated alignment of tiled sub-frames.

Main Results:

  • Zorro achieves reliable frame registration even with low dose and defocus.
  • The software effectively corrects specimen drift in cryo-EM movies.
  • Performance evaluation methods confirm Zorro's robustness.
  • Alignment of tiled sub-frames is successfully demonstrated.

Conclusions:

  • Zorro provides a robust solution for drift correction in cryo-EM dose fractionation.
  • The software facilitates improved structure resolution by accurate image alignment.
  • Zorro enhances the reliability of cryo-EM data processing.