Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrinsic regeneration mechanisms of murine lacrimal gland epithelial cells for therapeutic applications in aqueous-deficient dry eye disease.

The ocular surface·2026
Same author

Inflammation and Colorectal Cancer Pathogenesis: Molecular, Immunological, and Environmental Features for Therapy Response and Resistances.

International journal of molecular sciences·2026
Same author

Enhanced clinical assessment of Orofacial Clefts using interactive 3D visualization from Cinematic Rendering.

Dento maxillo facial radiology·2026
Same author

Priming neuronal regeneration: early adaptive mechanisms in zebrafish CNS injury.

Frontiers in molecular neuroscience·2026
Same author

Multimodal mechanical characterization pipeline for spinal cord tissue.

Acta biomaterialia·2026
Same author

PARK7 (DJ-1) indicates favorable prognosis and correlates with estrogen-receptor status and N<sup>ε</sup>-carboxymethyl-lysine (CML) accumulation in breast cancer.

Cancer treatment and research communications·2026
Same journal

Distinct cellular and subcellular distributions of the alpha and beta isoforms of Heat Shock Protein 90 in the mouse cerebral cortex.

Gene expression patterns : GEP·2026
Same journal

Molecular characterization and expression dynamics of Labeo rohita MDA5, its adaptor MAVS and pro-inflammatory cytokines against PAMPs stimulation and pathogen challenge.

Gene expression patterns : GEP·2026
Same journal

Defining the spatiotemporal expression pattern of Rap1 in the chicken embryonic spinal cord.

Gene expression patterns : GEP·2026
Same journal

Expression analysis of the TMC gene family in the auditory system during zebrafish embryonic development.

Gene expression patterns : GEP·2026
Same journal

Mapping the locations of barx1-expressing prechondrogenic condensation domains in the posterior arches during ceratobranchial cartilage formation in zebrafish.

Gene expression patterns : GEP·2026
Same journal

cDNA cloning, expression analysis, and subcellular localization of TRIM13 gene in zebrafish.

Gene expression patterns : GEP·2026
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.6K

Temporal and spatial expression pattern of Nnat during mouse eye development.

Saadettin Sel1, Eva Patzel1, Lucia Poggi1

  • 1Department of Ophthalmology, University Heidelberg, Germany.

Gene Expression Patterns : GEP
|January 1, 2017
PubMed
Summary
This summary is machine-generated.

Neuronatin (Nnat) is expressed in the developing and adult mouse eye, including the neuroretina, cornea, and lens. This suggests Nnat plays a role in eye development and maintenance.

Keywords:
DevelopmentEyeMouseNeuronatinNnat

More Related Videos

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
09:30

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

44.0K
Live-imaging of the Drosophila Pupal Eye
09:54

Live-imaging of the Drosophila Pupal Eye

Published on: January 12, 2015

10.2K

Related Experiment Videos

Last Updated: Mar 9, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.6K
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
09:30

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

44.0K
Live-imaging of the Drosophila Pupal Eye
09:54

Live-imaging of the Drosophila Pupal Eye

Published on: January 12, 2015

10.2K

Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Neuroscience

Background:

  • Neuronatin (Nnat) was first identified as a gene with high expression in the neonatal mammalian brain.
  • This study investigates the expression patterns of Nnat in the context of ocular development and in adult eyes.

Purpose of the Study:

  • To analyze the spatial and temporal expression patterns of Neuronatin (Nnat) during mouse eye development.
  • To determine the presence and localization of Nnat in various ocular tissues throughout development and in adulthood.

Main Methods:

  • Gene expression analysis using reverse transcription-polymerase chain reaction (RT-PCR) for Nnat transcripts.
  • Protein expression analysis via Western blot and immunohistochemistry.
  • Evaluation across multiple developmental stages: embryonic days (E) 12, 15, 16, and postnatal days (P) 7, 14, 30, and 175 (adult).

Main Results:

  • Nnat expression was observed in the embryonic and adult neuroretina.
  • Nnat protein and mRNA were detected in corneal epithelial, stromal, and endothelial cells.
  • Expression was also found in the lens epithelium, starting from E12 and continuing into adulthood.

Conclusions:

  • The identified expression pattern of Nnat in the eye suggests a significant role in ocular development.
  • Nnat may also be crucial for the maintenance of the mature eye structure and function.