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Related Concept Videos

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
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Visualizing polymeric bioresorbable scaffolds with three-dimensional image reconstruction using contrast-enhanced

Sheng Tu1, Fudong Hu2, Wei Cai1

  • 1Department of Cardiology, Fujian Medical University Union Hospital, Fujian Institute of Coronary Heart Disease, Fuzhou, Fujian, People's Republic of China.

The International Journal of Cardiovascular Imaging
|January 1, 2017
PubMed
Summary

Contrast-enhanced micro-computed tomography (mCT) can visualize polymeric bioresorbable scaffolds (BRSs). Optimal imaging requires over 2 hours of contrast agent soaking time for clear 3D reconstruction of BRSs.

Keywords:
Bioresorbable scaffoldsContrast mediumMicro-computed tomographyThree-dimensional reconstruction

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Area of Science:

  • Biomaterials Science
  • Medical Imaging
  • Cardiovascular Engineering

Background:

  • Polymeric bioresorbable scaffolds (BRSs) are crucial in cardiovascular interventions.
  • Visualizing BRSs in situ remains challenging, hindering assessment of their integration and degradation.
  • Micro-computed tomography (mCT) offers high-resolution imaging but requires optimization for soft tissues and biomaterials.

Purpose of the Study:

  • To establish and validate a contrast-enhanced mCT method for visualizing polymeric BRSs.
  • To determine optimal imaging parameters, specifically contrast agent soaking time, for accurate 3D reconstruction.
  • To assess the feasibility of differentiating BRS struts from surrounding vascular structures.

Main Methods:

  • Poly-L-lactic acid (PLLA) BRSs were implanted in coronary bifurcation phantom models.
  • Samples underwent five treatments: baseline (saline) and four contrast-enhanced scans (0, 1, 2, and 3 hours soaking time).
  • Contrast-enhanced mCT was performed, and image quality was assessed based on scaffold strut detectability and 3D reconstruction success rates.

Main Results:

  • Contrast-enhanced mCT successfully discriminated BRS struts from the vascular lumen immediately.
  • Complete separation of BRS struts from the vascular wall required >2 hours of contrast agent soaking time (Treatments 3 and 4).
  • Scaffold strut detectability and 3D reconstruction success rates significantly improved with soaking times ≥2 hours, reaching over 97%.

Conclusions:

  • Contrast-enhanced mCT is a feasible technique for high-resolution 3D visualization of polymeric BRSs.
  • A contrast agent soaking time of at least 2 hours is essential for optimal separation of BRSs from surrounding tissues in phantom models.
  • This imaging approach holds promise for preclinical evaluation of BRS performance and degradation.