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TFPI-2 expression is decreased in bladder cancer and is related to apoptosis

Chenchen Feng1, Yatfaat Ho, Chuanyu Sun

  • 1Department of Urology, Huashan Hospital, Fudan University, Shanghai, PR China.

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
|January 1, 2017
PubMed
Abstract

Insights

Tissue factor pathway inhibitor-2 (TFPI-2) expression is reduced in bladder cancer, correlating with tumor progression and decreased apoptosis. Lower TFPI-2 levels may indicate a poorer prognosis, suggesting TFPI-2 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Bladder cancer is a significant global health concern.
  • Understanding the molecular mechanisms underlying bladder cancer progression is crucial for developing effective treatments.
  • Tissue factor pathway inhibitor-2 (TFPI-2) is a protein with potential roles in cancer biology.

Purpose of the Study:

  • To investigate the expression levels of TFPI-2 in bladder cancer tissues.
  • To determine the relationship between TFPI-2 expression and clinicopathological features of bladder cancer.
  • To explore the correlation between TFPI-2 and apoptosis in bladder cancer cells.

Main Methods:

  • Immunohistochemical (IHC) staining was used to assess TFPI-2 expression in 24 bladder cancer samples.
  • TUNEL assay was employed to evaluate the apoptotic index of bladder cancer cells.
  • Quantitative PCR and Western blot analyses were performed to validate IHC findings.

Main Results:

  • TFPI-2 expression was significantly decreased in bladder cancer, with reduced levels correlating with higher tumor grade and stage.
  • TFPI-2 expression showed a positive correlation with increased apoptosis and a negative correlation with the Ki67 proliferation index.
  • IHC results for TFPI-2 expression were consistent with quantitative PCR and Western blot data.

Conclusions:

  • TFPI-2 expression is downregulated in bladder cancer and inversely associated with tumor progression and apoptosis.
  • TFPI-2 may serve as a valuable biomarker for bladder cancer diagnosis and prognosis.
  • Enhancing TFPI-2 levels could represent a potential therapeutic strategy to combat bladder cancer.

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