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Screening for antitumor activity of various plant extracts on HeLa and C 4-I cell lines
Gul Ozcan1, Ozlem Dagdeviren Ozsoylemez, Gizem Akman
1Department of Biology, Istanbul University, Faculty of Science, Istanbul, Turkey.
Purpose:
Cancer is a long process that leads the organism to death and is associated with the normal cells acquiring the ability to divide permanently. Nowadays, the use of natural products in cancer therapy has a great importance. In addition, working with plants that are endemic to Turkey and determining the biological activities of these plant extracts, is extremely important due to the potential for new drug development. There is no comparative study available in the literature on the antitumor effects of Colchicum sanguicolle, a new found species of the genus Colchicum in Turkey, Crateagus microphylla, of the genus Crateagus and Centaurea antiochia of the genus Centaurea. In this study, we tried to demonstrate the antitumor effect of these plant extracts on HeLa and C 4-1 cells.
Methods:
Five different doses (0.001, 0.01, 0.05, 0.25 and 0.5 mg/ml) of the three plant types were prepared and applied for 24, 48 and 72 hrs on the cervical cancer derived cell lines. Subsequently, the growth rate was evaluated with the mitochondrial dehydrogenase enzyme method.
Results:
Colchicum sanguicolle extracts showed the most effective antitumor activity. For the Colchicum sanguicolle extract, the IC50 dose for HeLa cells was 0.01 mg/ml at 48 hrs, while for the C-4 I cells it was 0.001 mg/ml at 48 hrs. These results showed that C-4 I cells were more sensitive to the Colchicum sanguicolle extracts. Conclus?on: The results of from this study regarding the antitumor effect of plant extracts of endemic varieties of Turkey may have an important place in design and development of anticancer drugs and would make contributions to other studies to be conducted in this area.
Insights
Colchicum sanguicolle extracts demonstrated significant antitumor activity against cervical cancer cell lines. This study highlights the potential of Turkish endemic plants in developing novel anticancer drugs.
Area of Science:
- Pharmacology
- Oncology
- Botany
Background:
- Natural products are increasingly important in cancer therapy.
- Turkey harbors endemic plant species with potential for new drug development.
- A comparative study on the antitumor effects of specific Turkish endemic plants was lacking.
Purpose of the Study:
- To investigate the antitumor effects of three Turkish endemic plant extracts: Colchicum sanguicolle, Crateagus microphylla, and Centaurea antiochia.
- To evaluate the efficacy of these extracts on HeLa and C-4-1 cervical cancer cell lines.
Main Methods:
- Plant extracts were prepared at five different concentrations (0.001–0.5 mg/ml).
- Extracts were applied to HeLa and C-4-1 cells for 24, 48, and 72 hours.
- Cell growth inhibition was assessed using the mitochondrial dehydrogenase enzyme assay.
Main Results:
- Colchicum sanguicolle extracts exhibited the most potent antitumor activity.
- The IC50 for Colchicum sanguicolle was 0.01 mg/ml on HeLa cells and 0.001 mg/ml on C-4-1 cells at 48 hours.
- C-4-1 cells were more sensitive to Colchicum sanguicolle extracts than HeLa cells.
Conclusions:
- Colchicum sanguicolle extracts show significant potential as a source for anticancer drug development.
- These findings contribute to the understanding of ethnopharmacology and may guide future research on Turkish endemic flora for cancer therapy.
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