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Comparative transcriptomic analysis reveals phenol tolerance mechanism of evolved Chlorella strain
Lin Zhou1, Dujia Cheng2, Liang Wang3
1Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
Bioresource Technology
|January 2, 2017
Summary
An evolved microalgae strain, Chlorella sp. L5, tolerates high phenol concentrations by up-regulating antioxidant enzymes and carotenoid biosynthesis genes. This enhances its resilience and photosynthetic efficiency.
Area of Science:
- Environmental microbiology
- Algal biotechnology
- Molecular biology
Background:
- High phenol concentrations inhibit microalgal growth by inducing oxidative stress.
- An evolved strain, Chlorella sp. L5, demonstrates tolerance to 500 mg/L phenol.
Purpose of the Study:
- To elucidate the genome-scale tolerance mechanisms of Chlorella sp. L5 to high phenol concentrations.
- To compare the transcriptomic profiles of Chlorella sp. L5 and its parent strain, Chlorella sp. L3.
Main Methods:
- Comparative transcriptomic analysis between Chlorella sp. L5 and Chlorella sp. L3.
- Analysis of gene expression related to antioxidant enzymes, carotenoids, photosynthesis, and carbohydrate metabolism.
Main Results:
- Up-regulation of genes encoding antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase) and carotenoids (astaxanthin, lutein, lycopene) in Chlorella sp. L5.
- Upregulation of genes involved in Photosystem I (PS I), Photosystem II (PS II), photosynthetic electron transport chain, and starch biosynthesis.
- Experimental validation showing increased total carbohydrate content in Chlorella sp. L5 under phenol stress, consistent with transcriptomic data.
Conclusions:
- The enhanced tolerance of Chlorella sp. L5 to high phenol concentrations is attributed to the upregulation of antioxidant defense systems and carotenoid biosynthesis pathways.
- Upregulated photosynthetic and carbohydrate biosynthesis genes contribute to the strain's resilience and metabolic adaptation under phenol stress.
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