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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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Published on: August 26, 2018

Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells.

Oron Goldstein¹, Karin Meyer², Yariv Greenshpan¹

¹The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.

Stem Cell Reports

|January 3, 2017

Summary

This summary is machine-generated.

Hematopoietic stem cells (HSCs) generate all blood and immune cells. This study maps alternative splicing isoforms in mouse HSCs, revealing novel gene functions and enhancing stem cell research.

Keywords:

HSC ImmGen RNA-sequencing analysis adult stem cell splicing alternative splicing hematopoietic stem cell splicing-function unannotated splicing whole-transcriptome splicing

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Area of Science:

Stem cell biology
Molecular biology
Genomics

Background:

Hematopoietic stem cells (HSCs) are crucial for generating all blood and immune cell lineages.
Transcriptome data has identified key genes in HSCs, but alternative splicing (AS) adds complexity.
Understanding AS is vital for a comprehensive view of HSC function.

Purpose of the Study:

To delineate alternative splicing (AS) and identify expressed isoforms in mouse hematopoietic stem cells (HSCs).
To map the complete landscape of splicing isoforms in HSCs.
To explore how AS may alter gene function in adult stem cells.

Main Methods:

Analysis of microarray and RNA-sequencing data from mouse HSCs.
Differential expression analysis of splicing factors.
Comprehensive mapping of gene and splicing isoforms.

Main Results:

Identification of multiple isoforms for known HSC genes.
Discovery of unannotated splicing events with potential functional implications.
A complete map of splicing isoforms in mouse HSCs was generated.

Conclusions:

Alternative splicing significantly expands the functional repertoire of HSCs.
The isoform-level transcriptome provides new insights into HSC biology.
This work offers a new dimension for understanding adult stem cells.