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Related Experiment Video

Updated: Mar 9, 2026

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
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Published on: May 2, 2018

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Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics.

Wolfgang Keil1, Lena M Kutscher2, Shai Shaham2

  • 1Center for Physics and Biology, The Rockefeller University, New York, NY 10065, USA; Laboratory of Developmental Genetics, The Rockefeller University, New York, NY 10065, USA.

Developmental Cell
|January 3, 2017
PubMed
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Researchers developed a microfluidic device for observing Caenorhabditis elegans larval development over three days. This method allows high-resolution imaging and quantitative analysis of cellular processes during development.

Area of Science:

  • Developmental Biology
  • Microfluidics
  • Cellular Biology

Background:

  • Long-term studies of Caenorhabditis elegans larval development are challenging due to the need for continuous movement and limitations of current immobilization techniques.
  • Existing methods often perturb development or are unsuitable for early larval stages, hindering detailed observation.

Purpose of the Study:

  • To present a novel microfluidic device for high-spatiotemporal resolution, long-term observation of Caenorhabditis elegans larval development.
  • To enable simultaneous tracking of multiple larvae from hatching to adulthood without perturbing their development.

Main Methods:

  • A simple microfluidic device with microchambers for culturing Caenorhabditis elegans larvae.
  • Periodic immobilization of larvae via compression for high-quality imaging, including weak fluorescence signals.
Keywords:
Caenorhabditis elegansPDA neuronPVD neurondendritic arborizationlarval developmentlinker cell deathlong-term imagingmicrofluidicstransdifferentiationvulval development

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  • Combined Nomarski and multichannel fluorescence microscopy for detailed cellular process analysis.
  • Automated image registration for generating time-lapse movies of complex biological processes.

    • Main Results:

    • Simultaneous tracking of ten C. elegans larvae from hatching to adulthood (approx. 3 days).
    • Acquisition of cell-cycle statistics for C. elegans vulval development, a model for organogenesis.
    • Detailed study of cell-fate specification, cell death, and transdifferentiation during post-embryonic development.
    • Generation of time-lapse movies illustrating complex neural arborization.

    Conclusions:

    • The developed microfluidic device facilitates non-perturbing, high-resolution, long-term observation of C. elegans larval development.
    • This technique enables quantitative analysis of time-dependent cellular behaviors and developmental processes.
    • Opens new avenues for studying organogenesis and neural development in C. elegans.