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Mapping Sentinel Lymph Node Metastasis by Dual-probe Optical Imaging.

Xiangyu Yang1, Zhe Wang2, Fuwu Zhang2

  • 1Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing 210009, China;; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health (NIH), Bethesda, MD 20892, USA.

Theranostics
|January 3, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a dual-tracer imaging method using hyaluronic acid (HA) and antibodies to map sentinel lymph nodes (SLNs) and detect cancer metastases simultaneously, offering real-time intraoperative guidance for SLNB procedures.

Keywords:
EGFRHER2.hyaluronic acidmetastasisoptical imagingsentinel lymph node

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Medical Imaging

Background:

  • Sentinel lymph node biopsy (SLNB) is standard for staging various cancers.
  • Accurate intraoperative identification of metastatic lymph nodes remains a challenge.
  • Current methods often require waiting for biopsy results, delaying treatment decisions.

Purpose of the Study:

  • To develop a method for simultaneous intraoperative mapping of sentinel lymph nodes (SLNs) and detection of tumor metastases within them.
  • To provide real-time guidance for SLNB procedures, improving accuracy and efficiency.
  • To evaluate the efficacy of dual-tracer imaging using hyaluronic acid and targeted antibodies.

Main Methods:

  • Conjugating hyaluronic acid (HA) with a near-infrared fluorophore (Cy5.5) as an SLN mapping agent targeting LYVE-1.
  • Labeling antibodies (cetuximab, trastuzumab) with a near-infrared fluorophore (IRDye800) for tumor-specific detection.
  • Utilizing a mouse model with engineered human cancer cells for lymph node (LN) metastasis.
  • Performing sequential intravenous administration of labeled antibodies and local administration of Cy5.5-HA, followed by optical imaging.

Main Results:

  • Hyaluronic acid (HA) demonstrated binding to LYVE-1, confirming its suitability as a lymphatic mapping agent.
  • The 10K size of HA was optimized for migration and retention in lymph nodes.
  • Dual-tracer imaging successfully differentiated between non-metastatic, partially metastatic, and fully tumor-occupied LNs based on distinct signal patterns.

Conclusions:

  • Fluorophore-conjugated HA shows potential as an effective lymphatic mapping agent for SLNB.
  • Dual-tracer imaging combining lymphatic mapping and tumor-targeting agents can accurately identify metastases within SLNs.
  • This approach offers accurate, real-time intraoperative guidance, potentially reducing the need for delayed biopsy analysis.