Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Ca <sup>2+</sup> -Sensitivity of Contraction is Increased in the Left Atrium and Left Ventricle of Patients with Ischemic Heart Failure.

bioRxiv : the preprint server for biology·2026
Same author

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same author

Combined Heart-Lung Transplant for Pulmonary Artery Aneurysm Secondary to Pulmonary Arterial Hypertension.

JACC. Case reports·2026
Same author

Reviving formalin-fixed, paraffin embedded (FFPE) tissues for on-slide and multiscale correlative microscopy.

Scientific reports·2026
Same author

Biomolecular condensation of cMLCK enables myosin motor phosphorylation in the heart.

bioRxiv : the preprint server for biology·2026
Same author

Hearts may grow concentrically to balance ATP supply and demand and eccentrically to stabilize titin-based stress.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Mar 9, 2026

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.6K

A Protocol for Collecting Human Cardiac Tissue for Research.

Cheavar A Blair1, Premi Haynes2, Stuart G Campbell3

  • 1Department of Physiology, University of Kentucky, Lexington, KY.

The VAD Journal : the Journal of Mechanical Assisted Circulation and Heart Failure
|January 3, 2017
PubMed
Summary

This protocol details collecting human myocardium for research from organ donors and heart failure patients. It offers practical insights for establishing similar human cardiac tissue collection programs.

Keywords:
CardiacHeart failureHumanVentricular assist device

More Related Videos

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
07:25

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Published on: December 17, 2019

8.9K
Preparation of Human Myocardial Tissue for Long-Term Cultivation
10:58

Preparation of Human Myocardial Tissue for Long-Term Cultivation

Published on: June 2, 2022

5.9K

Related Experiment Videos

Last Updated: Mar 9, 2026

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.6K
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
07:25

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Published on: December 17, 2019

8.9K
Preparation of Human Myocardial Tissue for Long-Term Cultivation
10:58

Preparation of Human Myocardial Tissue for Long-Term Cultivation

Published on: June 2, 2022

5.9K

Area of Science:

  • Cardiovascular Research
  • Translational Medicine
  • Biobanking

Background:

  • Human cardiac tissue is crucial for understanding heart disease.
  • Previous research has been limited by sample availability.
  • A standardized protocol for human myocardium collection is needed.

Purpose of the Study:

  • To describe a protocol for collecting human myocardium for translational research.
  • To share practical experience and challenges encountered since 2008.
  • To provide guidance for other institutions establishing similar programs.

Main Methods:

  • Collection of human myocardium from organ donors and heart failure patients (heart transplant or ventricular assist device).
  • Detailed description of the consent process.
  • Discussion of protocol costs, issues, and practical difficulties.

Main Results:

  • Procured approximately 4000 samples from 230 patients.
  • Successfully implemented a protocol for human cardiac tissue biobanking.
  • Gained extensive practical experience in managing human tissue samples.

Conclusions:

  • Human myocardium collection is feasible and essential for cardiac research.
  • Sharing practical challenges and solutions facilitates protocol implementation.
  • This protocol serves as a valuable resource for researchers worldwide.