[Use of acridine orange in the estimation of lymphoid cell migration in an organism]

Lymphoid cell migration was studied on the model of their transplantation from animal-donors to recipients. The studied organs were crushed small in the saline solution. The number of nuclei-containing elements has been determined in the obtained suspensions. Smears have been prepared and studied with the help of luminescence microscope combined with phase contrast device. We have determined and counted the ratio of "acridine orange cells" glittering in ultra-violet rays to all visible in phase-contrast nuclei containing cells. Then we have found the percentage of the marked cells in the organ from the total number of the injected lymphocytes. The usage of the given technique shown the enhancement of thymus cell migration to the liver after partial hepatectomy.