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Updated: Mar 9, 2026

Preparation of Quality Inositol Pyrophosphates
Published on: September 3, 2011
Bacillus subtilis iolU encodes an additional NADP+-dependent scyllo-inositol dehydrogenase
Dong-Min Kang1, Kosei Tanaka2, Shinji Takenaka1,2
1a Department of Agrobioscience , Graduate School of Agricultural Science, Kobe University , Kobe , Japan.
Abstract:
Bacillus subtilis genes iolG, iolW, iolX, ntdC, yfiI, yrbE, yteT, and yulF belong to the Gfo/Idh/MocA family. The functions of iolG, iolW, iolX, and ntdC are known; however, the functions of the others are unknown. We previously reported the B. subtilis cell factory simultaneously overexpressing iolG and iolW to achieve bioconversion of myo-inositol (MI) into scyllo-inositol (SI). YulF shares a significant similarity with IolW, the NADP+-dependent SI dehydrogenase. Transcriptional abundance of yulF did not correlate to that of iol genes involved in inositol metabolism. However, when yulF was overexpressed instead of iolW in the B. subtilis cell factory, SI was produced from MI, suggesting a similar function to iolW. In addition, we demonstrated that recombinant His6-tagged YulF converted scyllo-inosose into SI in an NADPH-dependent manner. We have thus identified yulF encoding an additional NADP+-dependent SI dehydrogenase, which we propose to rename iolU.
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