Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Point and Frameshift Mutations01:30

Point and Frameshift Mutations

977
Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
977
Mutations in Microorganisms01:18

Mutations in Microorganisms

610
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
610
Viral Mutations00:36

Viral Mutations

39.8K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.8K
Mutations01:39

Mutations

94.4K
Overview
94.4K
Mutations01:35

Mutations

44.2K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
44.2K
Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

63.5K
In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
63.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expanding Xpert MTB/RIF Ultra and lateral flow urine lipoarabinomannan testing for diagnosis of tuberculosis among adults living with HIV admitted to hospitals in Tanzania and Mozambique (EXULTANT): a randomised controlled trial.

The Lancet. Infectious diseases·2026
Same author

Diagnostic Assays for TB Disease and Drug Resistance Detection-Advances and Latest WHO Recommendations.

Cold Spring Harbor perspectives in medicine·2026
Same author

Phosphatidylserine liposomes for <i>Mycobacterium abscessus</i> infections management in people with cystic fibrosis non-eligible for CFTR modulators.

Frontiers in immunology·2026
Same author

Clinical evaluation of a commercial culture-free targeted next-generation sequencing test for diagnosis of drug-resistant tuberculosis.

Microbiology spectrum·2025
Same author

Diagnostic algorithms for tuberculosis in Europe: insights from the European Reference Laboratory Network for Tuberculosis (ERLTB-Net).

The Lancet regional health. Europe·2025
Same author

<i>In vitro</i> exposure to clofazimine can select for delamanid and pretomanid resistance in <i>Mycobacterium tuberculosis</i>.

Antimicrobial agents and chemotherapy·2025
Same journal

Concurrent Multibacillary Lepromatous Leprosy with Autonomic Dysfunction and Cavitary Pulmonary Tuberculosis Complicated by Spontaneous Pneumothorax and Subcutaneous Emphysema.

International journal of mycobacteriology·2026
Same journal

Pulmonary Tuberculosis following Chemotherapy for Metastatic Testicular Germ Cell Tumor: Possible Reactivation of Latent Tuberculosis Infection.

International journal of mycobacteriology·2026
Same journal

Disseminated Mycobacterium immunogenum -associated Hemophagocytic Lymphohistiocytosis after Stem Cell Transplantation.

International journal of mycobacteriology·2026
Same journal

Recurrent Paradoxical Reactions in Cervical Tuberculous Lymphadenitis During and after Antitubercular Therapy in an Immunocompetent Patient.

International journal of mycobacteriology·2026
Same journal

A Rare Infection in a Patient with Systemic Lupus Erythematosus Treated with Mycophenolate Mofetil.

International journal of mycobacteriology·2026
Same journal

Performance of Molecular Diagnostics in Strengthening Tuberculosis Detection and Drug Resistance Surveillance: A Comparative Study with Conventional Methods.

International journal of mycobacteriology·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.2K

Reaching consensus on drug resistance conferring mutations.

Daniela M Cirillo1, Paolo Miotto1, Elisa Tagliani1

  • 1Emerging Bacterial pathogens Unit, San Raffaele Scientific Institute, via Olgettina 60, Milan, Italy.

International Journal of Mycobacteriology
|January 4, 2017
PubMed
Summary
This summary is machine-generated.

A new database, the Relational Sequencing TB Data Platform (ReSeqTB), standardizes mutations linked to drug-resistant tuberculosis (TB). This resource aids in developing rapid molecular tests for better treatment guidance and preventing further resistance.

Keywords:
Conferring mutationsDrug resistanceMolecular-basedRapid drug-susceptibility tests

More Related Videos

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

11.1K
A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors
12:40

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

Published on: December 7, 2014

15.2K

Related Experiment Videos

Last Updated: Jan 25, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.2K
Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

11.1K
A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors
12:40

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

Published on: December 7, 2014

15.2K

Area of Science:

  • Microbiology
  • Genetics
  • Public Health

Background:

  • Drug-resistant tuberculosis (TB) poses a significant global health threat, necessitating rapid diagnostics.
  • Existing data on Mycobacterium tuberculosis drug resistance mutations are fragmented and lack standardization.
  • Molecular-based susceptibility testing is crucial for guiding treatment and preventing resistance.

Purpose of the Study:

  • To establish a global, curated, and standardized data-sharing platform for Mycobacterium tuberculosis.
  • To compile and validate genotypic and phenotypic information on drug resistance in TB.
  • To support the development of rapid molecular drug-susceptibility tests.

Main Methods:

  • The Relational Sequencing TB Data Platform (ReSeqTB) consortium critically reviewed published data on mutations associated with drug resistance in M. tuberculosis.
  • A comprehensive list of globally prevalent mutations was graded based on their association with drug resistance.
  • Expert validation by international scientists and organizations was integral to the process.

Main Results:

  • ReSeqTB serves as a single repository for curated sequence and metadata on M. tuberculosis strains.
  • The platform utilizes a validated dataset of drug resistance-associated mutations as a starting point.
  • Ongoing accumulation of new sequence data will continuously enhance the database.

Conclusions:

  • ReSeqTB provides a vital resource for understanding and combating drug-resistant TB.
  • The platform facilitates collaboration among global researchers and public health agencies.
  • Standardized genotypic and phenotypic data are essential for programmatic drug-susceptibility testing and TB control.