Related Experiment Video
Updated: Mar 9, 2026
An Epithelial Abrasion Model for Studying Corneal Wound Healing
Published on: December 29, 2021
Immediate effects of diamond burr debridement in patients with spontaneous chronic corneal epithelial defects, light
Charlotte Dawson1, Carolina Naranjo2,3, Belen Sanchez-Maldonado2
1Ophthalmology Service, Royal Veterinary College, University of London, Hawkshead Lane, North Mymms, AL9 7TA, UK.
Purpose:
To evaluate immediate effects of diamond burr debridement (DBD) on the cornea of canine patients diagnosed with spontaneous chronic corneal epithelial defects (SCCEDs).
Animals Studied:
Eight client owned dogs with SCCEDs.
Methods:
Nine eyes from eight dogs with SCCEDs underwent superficial keratectomy (SK). The ulcerated area was divided into quadrants with a 300-micron restricted depth knife. Two of four quadrants underwent DBD for 40-60 s. A SK followed immediately. One burred section and one nonburred section were fixed with formaldehyde 10% and underwent light microscopy (LM). The remaining quadrants from five eyes were fixed with glutaraldehyde 2.5% and underwent transmission electron microscopy (TEM). Masked pathologists evaluated the samples. A student's paired t-test was used to analyze the data.
Results:
With LM all nonburred samples had a superficial stromal hyaline acellular zone (HAZ), seven of the burred samples had an intermittent HAZ and in two burred samples this zone was absent. The HAZ thickness of burred samples (1.062 ± 0.664 μm) was significantly thinner than that of the nonburred samples (4.309 ± 1.348 μm) (P < 0.0001). Transmission electron microscopy showed an absence of basement membrane and the presence of an amorphous, fine fibrillar material in the superficial stroma in nonburred samples. This material was intermittent or absent in burred samples.
Conclusion:
DBD significantly reduces the superficial stromal HAZ in SCCEDs. A reduction of its thickness may be responsible for the healing rates reported with DBD.
