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Updated: Jul 4, 2026

Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products
Published on: December 5, 2020
Isotryptophan from Antarctic Bacillus amyloliquefaciens Pc3: purification, identification, characterization, and
Pengfei Cui1, Wenbin Guo1,2, Xinhua Chen1,2
1a Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography , State Oceanic Administration , Xiamen , P.R. China.
Abstract:
Strain Pc3 was isolated from Antarctic seawater and identified as Bacillus amyloliquefaciens. A compound with antifungal activity was purified from the fermentation supernatant of B. amyloliquefaciens Pc3. Its structure was determined to be (S)-2-amino-3-(1H-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (NMR) and high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS). Isotryptophan exhibited good thermal stability and antifungal activity against several plant-pathogenic fungi with low minimum inhibitory concentrations. Therefore, the characterisation of isotryptophan from the Antarctic B. amyloliquefaciens Pc3 will facilitate its potential application in the control of plant-pathogenic fungal infection.

