Application of allflex conservation buffer in illumina genotyping

Area of Science:

• Animal genetics
• Genomic technologies
• Biotechnology

Background:

• The Tissue Sampling Technology (TST) system utilizes a novel liquid conservation buffer for collecting biological samples.
• Illumina BeadChip genotyping is a widely used method for high-throughput genetic analysis.
• Assessing the compatibility of new sample preservation methods with established genotyping platforms is crucial for advancing genomic research.

Purpose of the Study:

• To evaluate the efficacy of the liquid conservation buffer from the TST system for Illumina BeadChip genotyping.
• To determine the stability of the liquid conservation buffer over extended periods post-sample collection.
• To investigate the impact of replenishing the conservation buffer on genotyping success rates.

Main Methods:

• Bovine ear punches were collected using the Tissue Sampling Unit (TSU) and Total Tagger Universal (TTU) systems.
• Samples were genotyped on Illumina BeadChips after incubation periods ranging from 0 to 720 hours.
• A replenishment study assessed call rates after repeated buffer replenishment.
• Animal identity was confirmed using the International Society of Animal Genetics (ISAG) SNP panel.

Main Results:

• Samples collected with the TSU system achieved an average call rate of 0.993, while TTU samples averaged 0.953, both above the 0.85 threshold.
• The liquid conservation buffer maintained high call rates across extended incubation times.
• A slight decrease in call rate was observed only after the fourth buffer replenishment for both TSU and TTU samples.

Conclusions:

• The liquid conservation buffer in the TST system is suitable for Illumina BeadChip genotyping applications.
• The TST system provides a stable method for sample preservation, enabling downstream genomic analyses.
• This technology facilitates multiple experimental uses of a single tissue sample, preserving it for future research.