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Updated: Mar 9, 2026

Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
Pex35 is a regulator of peroxisome abundance
Ido Yofe1, Kareem Soliman2, Silvia G Chuartzman1
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Abstract:
Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
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