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Updated: Mar 9, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Drug sensitivity pattern of various Staphylococcus species isolated at a tertiary care hospital
Lavan Singh1, M P Cariappa2, N K Das3
1Senior Adviser (Pathology), Military Hospital Meerut, UP, India.
Background:
Staphylococcus aureus and other Staphylococcus species are important pathogenic organisms and are responsible for various hospital infections. These are the predominant organisms found in pus and blood culture isolates. Infections arising due to these bacterial isolates are difficult to treat because of developing multidrug resistance.
Methods:
Over a 1-year period at a tertiary care hospital laboratory, 524 Staphylococci species were isolated from pus, blood and urine samples and species-level identification was done.
Results:
S. aureus formed the predominant species (70.8%) followed by coagulase-negative Staphylococcus (CoNS) (29.20%). S. aureus (91%) was the main isolate from pus samples; however, CoNS was isolated in equally higher proportion in blood culture (63.58%). Among the CoNS, Staphylococcus hemolyticus was the main isolate (9.3%). β-Lactamase production, alteration of PBP and MLSB resistance were seen in variable degrees in different species.
Conclusion:
CoNS group of Staphylococci is becoming an important cause of infection at tertiary care centres. The increased multidrug resistance among various Staphylococcus species is a cause of great concern and requires adequate measures to prevent the spread of these microorganisms in the hospital and the community.
Insights
Staphylococcus aureus and coagulase-negative staphylococci (CoNS) are significant causes of hospital infections. Rising multidrug resistance in these bacteria poses a serious treatment challenge.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Staphylococcus species are primary causes of hospital-acquired infections.
- These bacteria are frequently isolated from pus and blood cultures.
- Multidrug resistance complicates treatment of staphylococcal infections.
Purpose of the Study:
- To identify and characterize Staphylococcus species isolated from clinical samples.
- To assess the prevalence of different Staphylococcus species in a tertiary care setting.
- To investigate antimicrobial resistance patterns in isolated staphylococci.
Main Methods:
- Prospective isolation of 524 Staphylococcus species from pus, blood, and urine over one year.
- Species-level identification of all isolates.
- Assessment of antimicrobial resistance mechanisms including beta-lactamase production, PBP alteration, and MLSB resistance.
Main Results:
- Staphylococcus aureus was the predominant species (70.8%), especially in pus samples (91%).
- Coagulase-negative staphylococci (CoNS) constituted 29.20%, with a higher proportion in blood cultures (63.58%).
- Staphylococcus hemolyticus was the most common CoNS isolate; various resistance mechanisms were observed.
Conclusions:
- Coagulase-negative staphylococci are an emerging cause of infections in tertiary care centers.
- Increasing multidrug resistance among Staphylococcus species is a significant concern.
- Effective measures are needed to prevent the spread of these resistant bacteria in healthcare settings and the community.
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