Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.