Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Xylem and Transpiration-driven Transport of Resources02:03

Xylem and Transpiration-driven Transport of Resources

27.3K
The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
27.3K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

92.3K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
92.3K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

11.0K
11.0K
Carbohydrate Metabolism01:36

Carbohydrate Metabolism

14.7K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
14.7K
Introduction to Carbohydrates01:34

Introduction to Carbohydrates

23.2K
Carbohydrates, proteins, and fats are the primary macronutrients in the human diet. However, carbohydrates are the most favored source of energy in the body. They can be found in a wide variety of foods, including whole grains, fruit, and vegetables, in various forms, such as sugars, starch, and dietary fiber. Based on their structure, carbohydrates are classified into three main classes— monosaccharides, disaccharides, and polysaccharides. The body's cells can only utilize simple...
23.2K
Membrane Carbohydrates01:30

Membrane Carbohydrates

7.6K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A simplified strategy for chest wall reconstruction: locoregional flaps as a reliable alternative in resource-limited settings.

BMC surgery·2026
Same author

Multitargeted synergistic mechanisms of Salvia miltiorrhiza and its bioactive compounds: A review.

Journal of ethnopharmacology·2026
Same author

Root development progression involves RALF peptide-mediated ROS signaling and pectin dynamics in rice.

Nature communications·2026
Same author

Tuning Xylan Polymerisation Enhanced Fibre Digestibility Without Biomass Loss in Sheepgrass (Leymus chinensis).

Plant biotechnology journal·2026
Same author

Delactylation of the tumor suppressor ARHGDIB drives metastasis and chemoresistance in bladder cancer.

Cell reports·2026
Same author

HDAC2 as a therapeutic target in bladder cancer: insights into the altered epigenetic regulation and lysine lactylation.

Journal of experimental & clinical cancer research : CR·2025

Related Experiment Video

Updated: Mar 9, 2026

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part II: Carbohydrates

Published on: March 12, 2010

31.2K

Carbohydrate Composition Analysis in Xylem.

Baocai Zhang1, Yihua Zhou2

  • 1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Methods in Molecular Biology (Clifton, N.J.)
|January 5, 2017
PubMed
Summary
This summary is machine-generated.

This study details a gas chromatography-mass spectrometry (GC-MS) method for analyzing poplar xylem cell wall carbohydrates. The technique accurately quantifies neutral monosaccharides and cellulose content in plant tissues.

Keywords:
Alditol acetateCelluloseGas chromatographyMass spectrometryMonosaccharideNeutral sugar

More Related Videos

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part I: Lignin
12:04

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part I: Lignin

Published on: March 11, 2010

33.3K
Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
10:47

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Published on: June 20, 2019

9.3K

Related Experiment Videos

Last Updated: Mar 9, 2026

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part II: Carbohydrates

Published on: March 12, 2010

31.2K
Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part I: Lignin
12:04

Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part I: Lignin

Published on: March 11, 2010

33.3K
Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
10:47

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Published on: June 20, 2019

9.3K

Area of Science:

  • Plant Biology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Xylem cell walls are rich in carbohydrates, crucial for plant structure and function.
  • Accurate carbohydrate composition analysis is vital for understanding plant physiology and biomass utilization.
  • Existing methods may require refinement for specific plant tissues like poplar xylem.

Purpose of the Study:

  • To establish a precise method for quantifying neutral monosaccharides and cellulose in poplar xylem.
  • To adapt and validate gas chromatography-mass spectrometry (GC-MS) for plant cell wall analysis.
  • To provide a reliable protocol for researchers studying poplar wood chemistry.

Main Methods:

  • Extraction of cell wall residues from poplar xylem tissues.
  • Derivatization of carbohydrates into alditol acetates for GC analysis.
  • Quantification using gas chromatography-mass spectrometry (GC-MS) with established standards.

Main Results:

  • The developed GC-MS method demonstrated high accuracy and repeatability for carbohydrate quantification.
  • Specific protocols for sample preparation and derivative generation were optimized.
  • The method successfully quantified major neutral monosaccharides and cellulose in poplar xylem.

Conclusions:

  • GC-MS provides a robust and accurate approach for poplar xylem carbohydrate analysis.
  • This method facilitates detailed investigation into plant cell wall composition.
  • The protocol can be applied to other woody tissues and plant species.