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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
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Structural Analysis of Cell Wall Polysaccharides Using PACE.

Jennifer C Mortimer1

  • 1Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885 Hollis Street, Emeryville, CA, 94608, USA. jcmortimer@lbl.gov.

Methods in Molecular Biology (Clifton, N.J.)
|January 5, 2017
PubMed
Summary

Plant cell walls contain complex polysaccharides that influence cell properties. The Polysaccharide Analysis using Carbohydrate gel Electrophoresis (PACE) method offers a quick way to analyze polysaccharide quantity and structure.

Keywords:
8-aminonaphthalene-1,3,6-trisulfonic acidCell wallsElectrophoresisFluorescent labellingGlycosyl hydrolasesPolysaccharidesXylan

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Plant cell walls are primarily constructed from intricate polysaccharide networks.
  • The specific composition and arrangement of these polysaccharides are critical for determining cell shape, function, and adhesion.

Purpose of the Study:

  • To introduce and describe the Polysaccharide Analysis using Carbohydrate gel Electrophoresis (PACE) technique.
  • To highlight PACE as a method for analyzing polysaccharide quantity and structure in plant cell walls.

Main Methods:

  • The study utilizes the PACE technique, a straightforward and rapid gel electrophoresis method.
  • This method allows for the quantitative and structural analysis of polysaccharides.

Main Results:

  • PACE provides a simple and fast approach for polysaccharide analysis.
  • The technique is effective in determining both the amount and structural characteristics of polysaccharides.

Conclusions:

  • The PACE technique is a valuable tool for researchers studying plant cell wall polysaccharides.
  • This method facilitates a deeper understanding of how polysaccharide composition impacts plant cell properties.