MiR-365 participates in coronary atherosclerosis through regulating IL-6.

Area of Science:

• Cardiovascular Biology
• Molecular Medicine
• Immunology

Background:

• Coronary atherosclerosis (AS) is a significant cardiovascular disease.
• The role of microRNAs, such as miR-365, in AS pathogenesis is under investigation.
• Interleukin-6 (IL-6) is implicated in inflammatory processes within AS.

Purpose of the Study:

• To investigate the role and mechanism of miR-365 in coronary atherosclerosis (AS).
• To examine the relationship between miR-365 expression and IL-6 levels in AS patients.

Main Methods:

• Quantitative reverse transcription PCR (QRT-PCR) for miR-365 and IL-6 mRNA expression.
• Western blot and ELISA for IL-6 protein expression.
• Analysis of samples from AS patients and healthy individuals, including blood and coronary plaque.

Main Results:

• IL-6 expression (mRNA and protein) was significantly increased in coronary plaque, blood monocytes, and serum of AS patients compared to controls.
• miR-365 expression was significantly decreased in AS patients.
• A significant inverse correlation was observed between miR-365 and IL-6 levels.

Conclusions:

• miR-365 expression is down-regulated in coronary atherosclerosis.
• IL-6 expression is up-regulated in coronary atherosclerosis.
• MiR-365 may regulate AS pathogenesis and immune response by targeting IL-6.