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Brain State Dependence of Hippocampal Subthreshold Activity in Awake Mice.

Brad K Hulse1, Evgueniy V Lubenov1, Athanassios G Siapas2

  • 1Division of Biology and Biological Engineering, California Institute of Technology, 1200 East California Blvd., Pasadena, CA 91125, USA.

Cell Reports
|January 5, 2017
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Summary

Brain state shifts modulate hippocampal neuron membrane potential, influencing network activity. These subthreshold dynamics, including depolarizing ramps, precede and enable ripple generation for distinct processing modes.

Keywords:
arousalbrain statehippocampuslarge irregular activitymembrane potentialneuromodulationripplessharp wavessmall irregular activitytheta

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Area of Science:

  • Neuroscience
  • Computational Neuroscience

Background:

  • Neocortical neuron activity and brain states are well-studied.
  • Hippocampal neuron subthreshold modulation by brain states remains poorly understood.

Purpose of the Study:

  • To systematically characterize the subthreshold modulation of hippocampal neurons (dentate granule cells and CA1 pyramidal neurons) by brain states in awake mice.
  • To investigate how these subthreshold dynamics relate to network events like ripples.

Main Methods:

  • Whole-cell recordings from hippocampal neurons.
  • Multisite extracellular recordings.
  • Behavioral measurements in awake mice.

Main Results:

  • Brain states significantly modulate average membrane potential, subthreshold fluctuation amplitude, and proximity to spike threshold in hippocampal neurons.
  • Rapid arousal variations correlate with membrane potential fluctuations.
  • Subthreshold dynamics, including depolarizing ramps, precede and coincide with network ripple events.

Conclusions:

  • Coordinated shifts in hippocampal neuron subthreshold dynamics underlie transitions between distinct network processing modes.
  • Subthreshold dynamics play a crucial role in preparing the hippocampus for network events like ripples.