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Updated: Mar 9, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
Published on: October 18, 2022
Road to the future of systems biotechnology: CRISPR-Cas-mediated metabolic engineering for recombinant protein
Amir Roointan1,2, Mohammad Hossein Morowvat1,3
1a Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies , Shiraz , Iran.
Abstract:
The rising potential for CRISPR-Cas-mediated genome editing has revolutionized our strategies in basic and practical bioengineering research. It provides a predictable and precise method for genome modification in a robust and reproducible fashion. Emergence of systems biotechnology and synthetic biology approaches coupled with CRISPR-Cas technology could change the future of cell factories to possess some new features which have not been found naturally. We have discussed the possibility and versatile potentials of CRISPR-Cas technology for metabolic engineering of a recombinant host for heterologous protein production. We describe the mechanisms involved in this metabolic engineering approach and present the diverse features of its application in biotechnology and protein production.
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