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Updated: Mar 9, 2026

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
Published on: June 15, 2018
MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins
Hana Uhlíková1, Martin Solanský1, Vendula Hrdinová2
1Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia.
Abstract:
Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins.
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