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Surface layer protein from Lactobacillus acidophilus NCFM inhibit intestinal pathogen-induced apoptosis in HT-29

Jun Meng1, Qiu-Xiang Zhang2, Rong-Rong Lu2

  • 1School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan Province 450001, China; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

International Journal of Biological Macromolecules
|January 7, 2017
PubMed

Insights

Lactobacillus acidophilus NCFM surface layer protein (SLP) protects intestinal cells from pathogen-induced apoptosis. This probiotic protein inhibits cell death via a mitochondria-dependent pathway, offering potential for treating gut infections.

Area of Science:

  • Microbiology
  • Cell Biology
  • Immunology

Background:

  • Intestinal pathogens can induce apoptosis (programmed cell death) in epithelial cells.
  • Lactobacillus acidophilus NCFM is a probiotic bacterium with potential health benefits.

Purpose of the Study:

  • To investigate the inhibitory effects of Lactobacillus acidophilus NCFM surface layer protein (SLP) on Escherichia coli and Salmonella-induced apoptosis in HT-29 cells.
  • To elucidate the mechanism underlying SLP's protective effects.

Main Methods:

  • Fluorescent microscopy to observe chromatin condensation.
  • Flow cytometry to quantify apoptosis.
  • Measurement of mitochondrial membrane potential and intracellular calcium levels.
  • Assay of caspase-9 and caspase-3 activation.

Main Results:

  • SLP alleviated pathogen-induced chromatin condensation.
  • SLP significantly reduced apoptosis induced by E. coli (46%) and Salmonella (48%).
  • SLP inhibited mitochondrial membrane potential reduction and calcium influx.
  • SLP decreased the activation of caspase-9 and caspase-3.

Conclusions:

  • Lactobacillus acidophilus NCFM SLP protects HT-29 cells from intestinal pathogen-induced apoptosis.
  • The protective mechanism involves the mitochondria-mediated pathway.
  • SLP shows potential for treating intestinal infections and applications in food, biological, and pharmaceutical industries.
Keywords:
ApoptosisEscherichia coliLactobacillus acidophilusSalmonella typhimuriumSurface layer proteins

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