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Updated: Mar 9, 2026

Detection and Isolation of Apoptotic Bodies to High Purity
Published on: August 12, 2018
Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
Georgia K Atkin-Smith1, Stephanie Paone1, Damien J Zanker1
1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
Abstract:
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.

