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Related Concept Videos

Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
9.4K
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

4.3K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
4.3K
Types Of Column Chromatography01:29

Types Of Column Chromatography

14.4K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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Related Experiment Video

Updated: Mar 9, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Separation techniques: Chromatography.

Ozlem Coskun1

  • 1Department of Biophysics, Canakkale Onsekiz Mart University, Canakkale, Turkey.

Northern Clinics of Istanbul
|January 7, 2017
PubMed
Summary
This summary is machine-generated.

Chromatography is a key biophysical method for separating and purifying mixtures. This technique allows for protein purification using various separation mechanisms and stationary phase types, with column chromatography being widely used.

Keywords:
Chromatographycolumn chromatographyprotein purification

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
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Area of Science:

  • Biophysical techniques
  • Analytical chemistry
  • Biochemistry

Background:

  • Chromatography is vital for separating, identifying, and purifying mixture components.
  • Protein purification relies on characteristics like size, charge, hydrophobicity, and binding affinity.
  • Various chromatography techniques exist, differing in stationary phase and separation mechanisms.

Approach:

  • Discusses various chromatography techniques based on molecular characteristics and stationary phase interactions.
  • Highlights column, thin layer, and paper chromatography as methods based on the stationary bed.
  • Emphasizes column chromatography as a prevalent protein purification technique.

Key Points:

  • Proteins are purified using chromatography based on distinct physical and chemical properties.
  • Four primary separation mechanisms are ion exchange, surface adsorption, partition, and size exclusion.
  • Column chromatography is a widely adopted method for protein purification.

Conclusions:

  • Chromatography is a versatile and essential tool in biophysics and biochemistry.
  • Understanding different chromatographic principles allows for effective protein purification.
  • The choice of chromatography technique depends on the specific separation needs.