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Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Mar 9, 2026

LED Thermo Flow — Combining Optogenetics with Flow Cytometry
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LED Thermo Flow — Combining Optogenetics with Flow Cytometry

Published on: December 30, 2016

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LED Thermo Flow - Combining Optogenetics with Flow Cytometry.

Kathrin Brenker1, Kerstin Osthof2, Jianying Yang3

  • 1Max-Planck Institute for Immunobiology und Epigenetics; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg; Centre for Biological Signaling Studies, BIOSS, University of Freiburg; brenker@ie-freiburg.mpg.de.

Journal of Visualized Experiments : Jove
|January 7, 2017
PubMed
Summary
This summary is machine-generated.

We developed the LED Thermo Flow, a low-cost device enabling optogenetics with flow cytometry. This innovation allows real-time analysis of light-sensitive proteins in thousands of cells, accelerating optogenetic tool discovery.

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Area of Science:

  • Optogenetics and Flow Cytometry
  • Cellular Signaling and Molecular Biology

Background:

  • Optogenetic tools offer precise control over cellular signaling pathways but are limited by light delivery methods, often relying on microscopy.
  • Flow cytometry provides high-throughput, single-cell resolution analysis, yet its integration with optogenetics remains underdeveloped.

Purpose of the Study:

  • To present a novel, low-cost device, the LED Thermo Flow, integrating optogenetics with flow cytometry.
  • To enable controlled illumination of cells during flow cytometric measurements for advanced optogenetic studies.

Main Methods:

  • Development of the LED Thermo Flow device for integration with common flow cytometers.
  • Illumination of cells with specific wavelengths, intensities, and temperatures during flow cytometric analysis.
  • Characterization of Dronpa protein photoswitching kinetics in real-time using the developed system.

Main Results:

  • The LED Thermo Flow device successfully integrates optogenetics and flow cytometry capabilities.
  • Real-time in vivo characterization of Dronpa protein photoswitching kinetics was achieved.
  • The system demonstrates adaptability for various optically controlled substances and flow cytometers.

Conclusions:

  • The LED Thermo Flow device significantly expands experimental possibilities in optogenetics.
  • This technology simplifies the discovery and development of novel optogenetic tools.
  • It facilitates high-throughput, real-time analysis of light-sensitive biological processes.