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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Related Experiment Video

Updated: Mar 9, 2026

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Mammalian Cell Tissue Culture Techniques.

Katy Phelan¹, Kristin M May²

¹Florida Cancer Specialists and Research Institute, Fort Myers, Florida.

Current Protocols in Molecular Biology

|January 7, 2017

Summary

This summary is machine-generated.

This guide details essential skills for maintaining mammalian cell cultures in molecular biology. It covers aseptic techniques, media preparation, passaging, cryopreservation, and cell counting for reliable research outcomes.

Keywords:

aseptic technique freezing cells medium passaging cells tissue culture

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Last Updated: Mar 9, 2026

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Published on: January 18, 2012

Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Cultured mammalian cells are fundamental tools in molecular biology research.
Maintaining cellular integrity, function, and viability in vitro is critical for experimental success.

Purpose of the Study:

To outline the fundamental techniques for preserving mammalian cell cultures.
To provide a practical guide for researchers on essential cell culture skills.

Main Methods:

Aseptic technique maintenance.
Preparation of specialized cell culture media.
Cell passaging and subculturing procedures.
Cryopreservation (freezing) and long-term storage protocols.
Recovery of frozen cell stocks.
Viable cell counting methodologies.

Main Results:

The appendix provides a comprehensive overview of essential cell culture maintenance skills.
It details the practical steps required for each technique, ensuring reproducibility.

Conclusions:

Mastery of these basic skills is imperative for successful and reproducible mammalian cell culture.
Proper cell culture preservation ensures the reliability of molecular biology studies.