Structure and regulation of P-450s in the rat P450IIA gene subfamily
Related Experiment Videos
F J Gonzalez1, T Matsunaga, K Nagata
1Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892.
Abstract:
The rat P450IIA subfamily was characterized at the protein, cDNA, and gene level. The purified IIA1 and IIA2 P-450s displayed distinct positional specificities toward hydroxylation of the prototype substrate testosterone. The IIA1 and IIA2 genes were also regulated differently during development; IIA1 was expressed within 1 week after birth in both males and females, and was specifically suppressed in males at puberty, while IIA2 was not expressed in females and was activated at puberty in males. The cDNA-deduced amino acid sequences of these enzymes showed 88% similarity with interspersed regions of high and low similarity indicative of former gene conversion events. Both the IIA1 and IIA2 genes contained nine exons.