Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid...

Area of Science:

• Pharmacology
• Analytical Chemistry
• Cancer Research

Background:

• Two isomeric flavones, flavone A and flavone B, exhibit antineoplastic activity against colon cancer cell lines.
• Previous studies focused on flavone extraction and quantification in rat plasma.

Purpose of the Study:

• To develop and validate modified methods for extracting and quantifying flavones A and B in rat colon tissue.
• To adapt existing high-performance liquid chromatography (HPLC) methods for tissue analysis.

Main Methods:

• Tissue homogenization of rat colon samples.
• PVDF membrane filtration of the supernatant.
• Quantification using a single calibration curve via HPLC.

Main Results:

• The modified method achieved good separation for both flavones.
• Linearity (r² ≥ 0.99), precision, and accuracy were demonstrated for both flavones.
• Concentrations detected: 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g flavone B at 6 hours post-dosing.

Conclusions:

• The modified extraction methods provide good separation, precision, and accuracy for flavones A and B in rat colon tissue.
• This validated method enables reliable quantification of these potentially therapeutic flavones in biological matrices.