Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Endospores and Sporulation01:20

Endospores and Sporulation

6.5K
Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...
6.5K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

599
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
599
Special Staining Techniques01:13

Special Staining Techniques

1.5K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.5K
Surface Appendages of Archaea01:23

Surface Appendages of Archaea

781
Archaeal surface appendages are highly specialized structures essential for environmental adaptation, encompassing roles in adhesion, biofilm formation, and motility. Among these appendages, pili and archaella stand out for their distinct morphologies and functionalities, enabling archaea to thrive in diverse and often extreme environments.Pili: Adhesion and Biofilm FormationPili are filamentous structures assembled from pilin protein subunits, primarily contributing to adhesion and biofilm...
781

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of clinical feature of IgG4 related disease].

Zhonghua yi xue za zhi·2016
Same author

Noninvasive measurement of renal oxygen extraction fraction under the influence of respiratory challenge.

Journal of magnetic resonance imaging : JMRI·2016
Same author

MicroRNA-103 suppresses tumor cell proliferation by targeting PDCD10 in prostate cancer.

The Prostate·2016
Same author

Clear cell carcinoma arising in previous episiotomy scar: a case report and review of the literature.

Journal of ovarian research·2016
Same author

A DNA tetrahedron-based molecular beacon for tumor-related mRNA detection in living cells.

Chemical communications (Cambridge, England)·2016
Same author

Long non-coding RNA MALAT-1 is downregulated in preeclampsia and regulates proliferation, apoptosis, migration and invasion of JEG-3 trophoblast cells.

International journal of clinical and experimental pathology·2016
Same journal

Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.

Applied microbiology and biotechnology·2026
Same journal

Enhancing reproducibility in mixing time determination of stirred tank reactors via automated analysis and standardized inter-laboratory trials.

Applied microbiology and biotechnology·2026
Same journal

Hybrid genome assembly and phenotypic assays reveal carbohydrate metabolism diversity in Lacticaseibacillus strains.

Applied microbiology and biotechnology·2026
Same journal

Ammonium and nitrate fluxes drive metabolic functional shifts in tap water biofilms.

Applied microbiology and biotechnology·2026
Same journal

Effects of controlled fermentation by Lactiplantibacillus plantarum PBS067 on Vitis vinifera L. leaf extract.

Applied microbiology and biotechnology·2026
Same journal

N-terminal lipidation enhances membrane interaction and antifungal activity of a Trematocine-derived decapeptide.

Applied microbiology and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens
07:42

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens

Published on: March 19, 2019

7.2K

Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future.

He Wang1, Yunxiang Wang2, Ruijin Yang3

  • 1Jiyang College, Zhejiang Agriculture and Forestry University, Zhuji, Zhejiang, 311800, China. wh2989@zafu.edu.cn.

Applied Microbiology and Biotechnology
|January 8, 2017
PubMed
Summary
This summary is machine-generated.

The Bacillus subtilis spore-surface display system offers enhanced stability and safety for displaying proteins. This review summarizes recent advances, critical factors, and future directions for this biotechnology technique.

Keywords:
ApplicationBacillus subtilisDisplay efficiencyFactorSporeSurface display

More Related Videos

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
08:58

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Published on: April 15, 2019

10.7K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.7K

Related Experiment Videos

Last Updated: Mar 9, 2026

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens
07:42

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens

Published on: March 19, 2019

7.2K
Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
08:58

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Published on: April 15, 2019

10.7K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.7K

Area of Science:

  • Biotechnology
  • Microbiology
  • Molecular Biology

Background:

  • Spore-surface display systems leverage microbial spores for displaying heterologous molecules.
  • Bacillus subtilis spores are widely utilized due to their inherent stability and safety advantages.
  • The technique has been successfully employed for over a decade for antigen and protein expression.

Purpose of the Study:

  • To comprehensively review recent advancements in Bacillus subtilis spore-surface display.
  • To highlight the benefits and critical factors influencing display efficiency.
  • To provide insights and suggestions for future research and applications.

Main Methods:

  • Review of recent scientific literature on Bacillus subtilis spore-surface display.
  • Analysis of studies focusing on expression systems and display efficiencies.
  • Synthesis of information on factors affecting spore display.

Main Results:

  • Bacillus subtilis spore-surface display offers superior stability and safety compared to other systems.
  • Key factors influencing display efficiency include protein characteristics and expression strategies.
  • Significant progress has been made in optimizing heterologous protein display.

Conclusions:

  • The Bacillus subtilis spore-surface display system is a robust platform with considerable potential.
  • Further research into optimizing display efficiency and expanding applications is warranted.
  • This technique holds promise for various applications in biotechnology and beyond.