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Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
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2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

2.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2.0K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.5K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.5K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

829
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
829
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.7K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
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Related Experiment Video

Updated: Mar 9, 2026

Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
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Accelerating two-dimensional nuclear magnetic resonance correlation spectroscopy via selective coherence transfer.

Qimiao Ye1, Lin Chen1, Wenqi Qiu1

  • 1Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, China.

The Journal of Chemical Physics
|January 9, 2017
PubMed
Summary

Selective coherence transfer (SECOT) accelerates 2D NMR spectroscopy by converting chemical shifts to spatial positions. This novel method enables fast and accurate qualitative and quantitative analyses in chemistry and biology.

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Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biophysical Chemistry

Background:

  • Nuclear magnetic resonance (NMR) spectroscopy is vital for chemical, biological, and medical analyses.
  • One-dimensional 1H NMR faces limitations due to overlapping resonances, hindering detailed analysis.
  • Two-dimensional (2D) 1H NMR enhances spectral resolution but requires lengthy acquisition times.

Purpose of the Study:

  • To develop a method for accelerating 2D NMR correlation spectroscopy acquisition.
  • To overcome the trade-off between spectral resolution and experimental time in 2D NMR.
  • To enable faster and more accurate qualitative and quantitative NMR analyses.

Main Methods:

  • Proposed a selective coherence transfer (SECOT) method.
  • Converted chemical shifts into spatial positions within the sample.
  • Employed an echo planar spectroscopic imaging module for data acquisition.
  • Generated 2D correlation spectra via 2D Fourier transformation.

Main Results:

  • Demonstrated the feasibility and effectiveness of SECOT under various magnetic field conditions.
  • Validated SECOT's performance in quantitative analyses across different sample concentrations.
  • Achieved accelerated acquisition of 2D correlation spectra.

Conclusions:

  • SECOT offers a promising approach to overcome spectral congestion in 2D NMR.
  • The method significantly reduces experimental time while maintaining accuracy.
  • SECOT holds potential for rapid, precise, and stable qualitative and quantitative investigations in diverse chemical systems.