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Related Experiment Video

Updated: Mar 9, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Performing Skin Microbiome Research: A Method to the Madness.

Heidi H Kong1, Björn Andersson2, Thomas Clavel3

  • 1Dermatology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA.

The Journal of Investigative Dermatology
|January 9, 2017
PubMed
Summary

Investigating the skin microbiome is crucial for understanding human health and disease. This review outlines essential standards and methods for high-quality skin microbiome research.

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Area of Science:

  • Microbiology
  • Dermatology
  • Genomics

Background:

  • The human microbiome, particularly the skin microbiome, plays a significant role in health and various cutaneous disorders like acne, psoriasis, and atopic dermatitis.
  • Standardized research methods are needed to advance the understanding of the skin microbiome's complex contributions to health and disease.

Purpose of the Study:

  • To review current standards and best practices for conducting high-quality skin microbiome research.
  • To highlight key considerations for study design, sample handling, and data analysis in skin microbiome investigations.

Main Methods:

  • Literature review of existing research standards and methodologies in skin microbiome studies.
  • Discussion of challenges unique to skin microbiome research, including sampling and processing.

Main Results:

  • Identified critical factors for standardized skin microbiome research: clinical study design, skin sampling techniques, sample processing, DNA sequencing methods, appropriate control inclusion, and robust data analysis.
  • Emphasized the need for common language and validated methods to ensure research quality and comparability.

Conclusions:

  • Standardization of methods and clear guidelines are essential for reliable and reproducible skin microbiome research.
  • Addressing skin-specific challenges is key to advancing our understanding of the skin microbiome in health and disease.