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Dynamic intravital imaging of cell-cell interactions in the lymph node
Jens V Stein1, Santiago F Gonzalez2
1Theodor Kocher Institute, University of Bern, Bern, Switzerland.
The Journal of Allergy and Clinical Immunology
|January 10, 2017
Summary
Live imaging using 2-photon microscopy reveals distinct cell behaviors in lymph nodes. This approach enhances understanding of immune cell interactions and immune response initiation in draining lymph nodes.
Area of Science:
- Immunology
- Cell Biology
- Microscopy
Background:
- 2-photon intravital microscopy has become a key tool for studying immune cell dynamics.
- Understanding cell interactions is crucial for deciphering immune functions.
- Lymph nodes are central sites for initiating adaptive immune responses.
Purpose of the Study:
- To review recent advancements in live imaging of lymph nodes.
- To categorize immune cells within lymph nodes based on motility.
- To highlight the contribution of in vivo imaging to understanding immune responses.
Main Methods:
- Application of 2-photon intravital microscopy for live imaging.
- Classification of immune cells into sessile and motile compartments.
- In vivo observation of cell behavior within draining lymph nodes.
Main Results:
- Identification of a sessile compartment (stromal cells, macrophages, resident dendritic cells) and a motile compartment (T and B lymphocytes).
- Demonstration of how live imaging visualizes distinct cell motility patterns.
- Correlation of observed cell behaviors with immune response initiation.
Conclusions:
- Live imaging provides critical insights into immune cell interactions within lymph nodes.
- The classification of cells into motile and sessile compartments aids in understanding immune regulation.
- In vivo microscopy is essential for advancing our knowledge of immune surveillance and response.
