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Molecular characterization of a p38 mitogen-activated protein kinase gene from Scylla paramamosain and its expression

Zehui Yu1, Yi Geng1, Anming Huang1

  • 1College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.

Journal of Invertebrate Pathology
|January 10, 2017
PubMed

Insights

A novel p38 MAPK gene was identified in S. paramamosain, showing wide tissue expression and increased levels upon bacterial and viral infection, highlighting its immune role.

Area of Science:

  • * Molecular Biology
  • * Immunology
  • * Crustacean Research

Background:

  • * The p38 MAPK signaling pathway is crucial for cellular responses to stress and pathogens.
  • * Understanding immune gene function in shrimp is vital for aquaculture and disease management.

Purpose of the Study:

  • * To clone and characterize a novel p38 MAPK gene from the shrimp S. paramamosain.
  • * To investigate the tissue distribution and expression patterns of the Sp-p38 MAPK gene.
  • * To elucidate the role of Sp-p38 MAPK in immune responses against bacterial and viral infections.

Main Methods:

  • * Rapid amplification of cDNA ends (RACE) technology for gene cloning.
  • * Phylogenetic analysis to determine evolutionary relationships.
  • * Quantitative expression analysis in various tissues and post-infection.

Main Results:

  • * A novel p38 MAPK gene (Sp-p38) was successfully cloned and characterized.
  • * Sp-p38 MAPK exhibited broad tissue distribution, with highest expression in hemocytes and intestines.
  • * Expression levels significantly increased following bacterial (S. aureus, V. harveyi) and WSSV infections.

Conclusions:

  • * The Sp-p38 MAPK gene plays a significant role in the immune defense of S. paramamosain.
  • * This study provides foundational data on Sp-p38 MAPK's biological characteristics and in vivo function.
  • * Findings contribute to understanding shrimp immunity against common pathogens.
Keywords:
CloningExpression profilesP38 mitogen-activated protein kinase genePathogen infectionScylla paramamosain

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